Bidstruplamm2074

In this ex vivo study, S. cervi parasitoses were treated with Ivermectin (50 μM), Albendazole (200 μM) alone and Ivermectin + Albendazole (50 + 200 μM) at 37°C for 8 h and the motility and viability of the parasitoses were evaluated. Individually both drugs Ivermectin (Iver) and Albendazole (Alb) are reported to affect the function and integrity of ER, however till date, no reports are available on the functional changes in ER due to a combined Iver and Alb treatment of bovine helminth parasitosis. Here, we report the lethal effect of a combination treatment of Iver and Alb against adult bovine filarial parasitosis Setaria cervi. The underlying mechanism of drug action was elucidated by performing a systematic biochemical, molecular and proteomics based study. Altered calcium homeostasis in drug treated parasitoses lead to reduction in levels of total Endoplasmic Reticulum (ER) calcium by 50 % and 61 % and elevation by 50 % and 63 % in cytosol in Iver alone and Iver + Alb treated parasitoses respectively. Furprotein folding were also found to be downregulated. Thus, this study based on biochemical and proteomic approaches indicates that a combination of anti-filarial drugs Iver and Alb can alter calcium homeostasis in bovine filarial parasitosis leading to induction of ER stress culminating into apoptosis.Coronocyclus labiatus and Cylicodontophorus bicoronatus are two significant horse parasitic nematodes which are classified into subfamily Cyathostominae, family Strongylidae, however, the classification of these nematodes has been controversial for more than a century. Mitochondrial (mt) genomes are considered valuable sources for parasite taxonomy, population genetics, and systematics studies. In the present study, the mt genomes of Co. labiatus and Cd. bicoronatus (type species) were determined and subsequently compared with those from closely related species by phylogenetic analysis based on concatenated datasets of amino acid sequences predicted from mt protein-coding genes. The complete mt genomes of Co. labiatus and Cd. bicoronatus were circular with 13,827 bp and 13,753 bp in size, respectively. Both mt genomes consisted of a total of 12 protein-coding genes, 22 transfer RNA genes, two ribosomal RNA genes and two non-coding regions. All protein coding genes were transcribed in the same direction, and t a distinct branch with Cyathostominae species, which was closer to Triodontophorus than Poteriostomum imparidentatum. We concluded Coronocyclus might be closely related with Cyathostomum but represent a distinct genus based on comparative mt genome sequences and phylogenetic analyses. The availability of complete mt genome sequences of Co. labiatus and Cd. bicoronatus provides new and useful genetic markers for further studies on Strongylidae nematodes.Trypanosoma evansi is an extracellular flagellate blood protozoan parasite and an etiological agent of animal trypanosomosis. Presently, only a few drugs are registered and have been used for the treatment of animal trypanosomosis, but they show severe toxic effects and also face the problem of drug resistance. Naphthoquinones (NTQ) are considered as fortunate structures in the field of medicinal chemistry as they have been reported for their antitrypanosomal potential against other trypanosomes-T. brucei and T. cruzi. In the present study, six naphthoquinones (NTQ1-NTQ6) derivatives were evaluated for anti-trypanosomal activity by demonstrating their growth inhibitory effect against T. evansi. All NTQs significantly (p less then 0.001) exhibited activity against parasite growth and multiplication with IC50 values of 11.48 μM, 373.6 μM, 12.97 μM, 21.97 μM, 18.19 μM and 5.758 μM but NTQ1, NTQ3 and NTQ6 were selected based on their IC50 value for further studies. The dose-and time-dependent morphological effect on parasite was evaluated including the measurement of reactive oxygen species (ROS) by spectrofluorometery and measurement of apoptosis by flow cytometry. The selected NTQs exhibited a significant production of ROS and displayed a significant AV+ and PI+ labelled cells in the axenic culture of T. evansi than quinapyramine methyl sulphate (QPS), as reference control. NTQs also showed more cytotoxic effect on horse peripheral blood mononuclear cells as compare to QPS. Therefore, we confirmed the antitrypanosomal activity and apoptotic-like mechanism of NTQs in an axenic culture of T. evansi.

The precise planning of metatarsal (MT) I length in hallux valgus surgery is important. However, currently no tool exists which allows the surgeon to reliably predict this parameter.

30 virtual 3-dimensional hallux valgus surgeries were performed on varied deformation models based on cadaveric feet scans. The shortening of the first ray during distal metatarsal I osteotomy for different osteotomy angles were measured. An algebraic 2-dimensional calculation was done and compared to the results obtained from the 3-dimensional models.

Inadvertent shortening of the first metatarsal bone can be as much as 8 mm depending on the amount of intermetatarsal angle (IMA) correction and osteotomy angle. Comparison of the 3 dimensional simulations and the 2 dimensional model resulted in a very strong correlation (R > 0.99 p < 0.00001). Based on our findings an anterior pointing osteotomy of approximately 10° is necessary to restore the length in distal metatarsal I hallux valgus surgery.

A slight misdirectioncedure through exact orientation of the osteotomy angle. If no alteration of length is intended, it may be generalized that an anterior direction of the cut relative to the second metatarsal bone will preserve the length of the first metatarsal bone.The preparation of fast, highly responsive and reliable gas sensing devices for the detection of acetone gas is considered to be a key challenge for the development of accurate disease diagnosis systems through exhaled respiratory gases. In the paper, yolk shell Sb2O3/WO3 is synthesized and its gas sensing performance was studied by static test system. Special, the maximum response value of 11 Sb2O3/WO3 yolk-shell (WO3-1 YSL) sensor to 100 ppm acetone can reach as high as 50.0 at 200 ℃. And it also exhibits excellent response/recover time (4 s/5 s), low detection limit (2 ppm) and superior selectivity towards acetone. More importantly, in mixed selective gas test, the sensor shows high selectivity towards acetone. And the mechanism is analyzed by ex-situ XPS. The excellent gas-sensing performance can be attributed to unique yolk-shell structure, which facilitates the rapid transport of charge carriers from the surface to the bulk and provides more active sites for gas adsorption and desorption; the heterojunction between of Sb2O3 and WO3, which promotes oxygen pre-adsorption on the surface and increasing the interfacial potential; the increased oxygen vacancies which allowing more chemisorbed oxygen to form.Capturing virus aerosols in a small volume of liquid is essential when monitoring airborne viruses. As such, aerosol-to-hydrosol enrichment is required to produce a detectable viral sample for real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) assays. To meet this requirement, the efficient and non-destructive collection of airborne virus particles is needed, while the incoming air flow rate should be sufficiently high to quickly collect a large number of virus particles. To achieve this, we introduced a high air flow-rate electrostatic sampler (HAFES) that collected virus aerosols (human coronavirus 229E, influenza A virus subtypes H1N1 and H3N2, and bacteriophage MS2) in a continuously flowing liquid. Viral collection efficiency was evaluated using aerosol particle counts, while viral recovery rates were assessed using real-time qRT-PCR and plaque assays. An air sampling period of 20 min was sufficient to produce a sample suitable for use in real-time qRT-PCR in a viral epidemic scenario.The content of toxic heavy metals (THMs), including lead (Pb), arsenic (As), cadmium (Cd), and mercury (Hg), was determined in a total of 10,245 samples for 279 types of Chinese herbal medicine (CHM) using a validated inductively coupled plasma mass spectrometry method. The exceeding rate (ER) for the four THMs were calculated based on diverse permissible limits (PLs) established by different organizations and national pharmacopeias. Cluster analysis was used to classify the degree risk of THMs contamination according to the calculated ER. Results revealed that Cibotii rhizome, Selaginellae herba, Morindae officinalis radix, Asprellae ilicis radix, and Toxicodendri resina exhibited high-degree risk of Pb contamination. TMP269 Eckloniae/Laminariae thallus, Spirodelae herba, and Naturalis indigo possessed high-degree risk of As contamination. Tetrapanacis medulla, Centipedae herba, Cyathulae radix, Linderae radix, Meretricis/Cyclinae concha, and Tabanus displayed high-degree risk of Cd contamination. Toxicodendri resina has high-degree risk of Hg contamination. In addition, six types of CHM, including Asprellae ilicis radix, Toxicodendri resina, Eckloniae/Laminariae thallus, Fossilia Ossis Mastodi, Haematitum, and Hedyotidis diffusae herba, may have non-carcinogenic health risk after consumption of raw materials because the calculated hazard quotient and hazard index were over 1.0. In summary, these data provide useful information about THMs contamination in CHM.Quantum chemistry combined with kinetic simulation and drop tube furnace (DTF) experiments were conducted to reveal the transformation behavior of chromium at the presence of steam and HCl under oxy-coal combustion. A completed kinetic system Cr‒O‒H‒Cl containing 107 elementary reactions was firstly proposed. The unknown microcosmic reaction paths and corresponding Arrhenius parameters were calculated via quantum chemistry. Kinetic simulations on the basis of Cr‒O‒H‒Cl system clarified that HCl promoted the oxidation of chromium to hexavalent CrO2Cl2. Coexistence of HCl and steam divided the transition of chromium into two stages. At the early stage, reaction rate between chromium and steam was faster than chromium with HCl, chromium mainly transformed to CrO(OH)2. Hereafter, HCl was dominant in the transformation of chromium, then chromium mainly presented as CrO2Cl2. Moreover, DTF experimental results indicated that introduction of HCl into combustion atmosphere induced more chromium release. Presence of steam reinforced the effect of HCl on chromium attributing to the significant transition of CrOx(OH)y to CrO2Cl2. Although CaO and Fe2O3 both exhibited good reactivity with chromium, presence of HCl largely suppressed chromium capture by CaO and Fe2O3. Moreover, the inhibition effect of HCl on Fe2O3 was stronger than CaO for Cr capture.Laccases are multicopper containing oxidase enzymes that are highly important in environmental remediation and biotechnology. To date, complex Copper containing materials have been reported as laccase mimic, and the possibility of a non-Cu laccase mimic remained unknown. In this work, we report an exceptionally simple functional laccase mimic based on coral-like silver citrate (AgCit) microstructures. The AgCit was synthesized by a simple precipitation method and was found to possess excellent laccase-like activity capable of oxidizing phenolic substrates and the endocrine hormone adrenaline. Compared to the natural laccase enzyme, our reported laccase-mimic has a higher υmax and lower Km value using adrenaline as a substrate. In addition, the AgCit laccase mimic was observed to be stable at extreme pH, higher temperature, and suitable for long-term storage at room temperature. The laccase-like properties of the AgCit nanozyme were successfully applied for the quantification and degradation of various phenolic pollutants and the adrenaline hormone.