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Survivorship at 1 dph and 16 dph was 24% and 54% higher, respectively, under elevated CO2 compared with ambient conditions. Elevated temperature increased survival (24%) at 1 dph, but not at 16 dph. These results suggest that projected climate change scenarios may have an overall positive effect on early life history growth and survival in this population of C. auratus. This could benefit recruitment success, but needs to be weighed against negative effects of elevated CO2 on metabolic rates and swimming performance observed in other studies on the same population.Macrophytes play an important structural and functional role in marine ecosystems but are experiencing a considerable decline in many areas of the Mediterranean Sea. Despite the long tradition of studies on vegetated marine habitats in the Gulf of Naples (Italy), a gap of knowledge on their long-term dynamics has recently been highlighted, mainly in the most anthropised areas. This work aimed to provide insights from the historic industrialised coastal area of the Site of National Interest (SIN) Bagnoli-Coroglio (Gulf of Pozzuoli, Italy), after decades of chemical contaminations and coastal transformation, to build a baseline for the next remediation and restoration programs. Historical occurrence of macroalgae and seagrasses before, during and after the industrial period was assessed; in particular, we focused the attention mainly on habitat-forming species, due to the vital function played in the trophic net. We observed no differences in the macroalgal diversity between the SIN district and the other parts of the Gulf of Pozzuoli, except for the Gulf of Pozzuoli during the industrial period, where the biodiversity showed a decreasing trend. However, a substantial regression of the largest macrophytes (Fucales) on rocky coasts and of marine monocots on sandy bottoms, all over the area, has been recorded. A loss of about 70% has concerned seagrass cover, mainly Posidonia oceanica, in the contaminated area of the SIN at present, this engineering species is missing on the bottom, facing the industrialised area. The human-made coastline transformation, the lack of natural substrates and the spatial remoteness among donor populations seem to be the leading causes of the significant decrease, over time, of marine forests in this study area. These factors should be taken into account to establish conservation priorities and for plant restoration.The use of sexual propagules to restore seagrass meadows has raised increasing attention in the last years as seed-based strategies avoid impacts on donor beds while preserving genetic diversity in restored populations. selleck chemicals llc However, the availability of suitable microsites for seedling establishment at transplantation locations is crucial in order to achieve positive outcome of restoration actions. In this study we develop ad-hoc holders that act as optimal microsites for Posidonia oceanica seedling establishment. Holders are intended to be transferred in the field for restoration purposes after few months of indoor seedling culture. Seedling ability to self-anchor to rocky substrates via adhesive root hairs was exploited. We tested rocky holders with different designs in order to maximize seedling survival and settlement. The effect of the holder design on seedling anchorage performances was evaluated. Holders were provided with different topographical complexity and substrate slope. Topographical complexity significantly influenced settlement success, as seedlings did not attach to flat holders, while anchorage reached 100% on holders provided with complexity at seed and the root scales. Substrate slope did not affect the percentage of anchored seedlings, conversely it influenced root growth pattern and thus anchorage stability. This study shows how ecological knowledge of species' life history strategies and associated critical traits provides valuable hints to develop alternative approaches to seagrass restoration tailored to the biology of the system under study.

The primary aim of this study is to compare the clinical course and laboratory parameters of acute cholecystitis in patients with diabetes vs. patients without diabetes.

The study involved patients who underwent emergency cholecystectomy in the Department of General, Endocrine and Transplant Surgery of University Clinical Center in Gdansk (Poland) between 2007 and 2017. There were 267 patients included in the study. The control group of 197 patients was age and sex matched at a 31 ratio. The following was compared between the groups symptoms at admission, course of surgery, postoperative course, length of hospitalization, total costs of hospitalization and antibiotic therapy, other than routine perioperative prophylaxis.

There was no significant difference between the patients with and without diabetes regarding symptoms at admission. Operative and postoperative complication rates were significantly higher in the patients with diabetes. The operative time and length of hospitalization were significantly longer in the study group. The conversion rate was not higher in the study group, but classic surgery was performed significantly more often. The patients without diabetes had less pronounced symptoms with more locally advanced disease.

Our study demonstrates that patients with diabetes have a significantly more eventful course of acute cholecystitis than patients without diabetes. Patients with diabetes should therefore be qualified for cholecystectomy early in the course of acute cholecystitis.

Our study demonstrates that patients with diabetes have a significantly more eventful course of acute cholecystitis than patients without diabetes. Patients with diabetes should therefore be qualified for cholecystectomy early in the course of acute cholecystitis.Reading acquisition involves learning to associate visual symbols with spoken language. Multiple lines of evidence indicate that instruction on the relationship between spellings and sounds may be particularly important.However, it is unclear whether the effectiveness of this form of instruction depends on pre-existing oral language knowledge.To investigate this issue, we developed a series of computational models of reading incorporating orthographic, phonological and semantic processing to simulate bothartificialand natural orthographic learning conditions in adults and children. We exposed the models to instruction focused on spelling-sound or spelling-meaning relationships, and tested the influence of the models' oral language proficiency on the effectiveness of these training regimes. Overall, the simulations indicated thatoral language proficiency is a vital foundation for reading acquisition, and may modulate the effectiveness of reading instruction. These results provide a computational basis for the Simple View of Reading,and emphasise the importance of both oral language knowledge and spelling-sound instructionin the initial stages of learning to read.