Patricksnyder6767

Three new species of virgulate xiphidiocercariae infecting the freshwater snails of Malabar, Kerala is described. Cercaria sp. XXVI Malabar n. sp. recovered from Bellamya bengalensis collected from Malappuram district of Kerala is characterized by an elongate-oval, spinose body and a highly contractile and spinose tail; dagger-shaped stylet with reinforced walls; coma-shaped virgula organ; muscular pharynx; short, narrow oesophagus; three pairs of penetration glands; bean-shaped excretory bladder and 12 pairs of flame cells. Cercaria sp. XXVII Malabar n. sp. recovered from Thiara tuberculata collected from Wayanad district of Kerala has a round to elongate-oval, spinose body; a highly contractile, spinose tail; dagger-shaped stylet; muscular pharynx; short oesophagus; narrow caeca extending up to ventral sucker; three pairs of penetration glands; bicornuate, thin-walled excretory bladder, and 12 pairs of flame cells. Cercaria sp. XXVII Malabar n. sp. infecting Paracrostoma huegelii also collected from Wayanad district, Kerala is characterized by elongate-oval, spinose body; contractile and spinose tail; dagger-shaped, inwardly curved stylet; virgula organ with elongated lobes; globular pharynx; three pairs of penetration glands; bicornuate, transversely elongate excretory bladder and 18 pairs of flame cells. All the three cercariae developed in sporocysts within the heapatopancreatic tissues of their respective snail hosts. The cercariae were compared with related species to establish their systematic position.This communication deals with the morphology, taxonomy and life history of six new acephaline gregarines (Apicomplexa Gregarinomorphea) of the genus Monocystis Stein (1848), Nematocystis Hesse (1909), Aikinetocystis Gates (1926) and Dirhynchocystis Cognetti de Martiis (1921) from oligochaete hosts. These are Monocystis molnari sp. nov. from Metaphire anomala (Clitellata Opisthopora Megascolecidae); Monocystis paneki sp. nov. from Lampito mauritii (Clitellata Opisthopora Megascolecidae); Monocystis bulbae sp. nov from Metaphire posthuma (Clitellata Opisthopora Megascolecidae); Nematocystis cylindrum sp. nov. from Perionyx excavatus (Clitellata Opisthopora Megascolecidae); Aikinetocystis perionyxae sp. nov. from Perionyx excavatus (Clitellata Opisthopora Megascolecidae) and Dirhynchocystis drawidae sp. nov. from Drawida nepalensis (Clitellata Haplotaxida Moniligastridae). Trophozoites of Monocystis molnari sp. nov. elongated and cylindrical. Gametocysts elliptoid. Oocysts fusiform. Trophozoites of Monocystis paneki sp. nov. elongated and hump like. Gametocysts orbicular. Oocysts fusiform. Trophozoites of Monocystis bulbae sp. nov. elongated and cylindrical. Gametocysts fusiform. Oocysts fusiform. Trophozoites of Nematocystis cylindrum sp. nov. cylindrical. Gametocysts orbicular. Oocysts fusiform. Trophozoites of Aikinetocystis perionyxae sp. nov. cylindrical. Gametocyst ovoid. Oocysts fusiform. Dirhynchocystis drawidae sp. nov. having a capsule like body. Gametocysts orbicular. Oocysts rhomboid.Since Cutaneous leishmaniasis is a problematic parasitic infection in the west of Iran, this study was carried out to clarify the epidemiological aspects of Cl. Our database includes all Cl cases with detailed information during 2014-2018 in Ilam province, west of Iran. Patients referred to health and clinical centers in our study areas then health providers and laboratory technicians took smears to detect leishmania parasites under light microscope. SPSS24 software used for analyzing data by the Chi square test. Mehran and Dehloran districts were alone accounted for 50% of all reported cases. ≥ 36 age group was dominant cases also housekeepers were at the top of categories in case of occupation. Hand and leg entangled with this infection more than other organs. Moreover, most cases observed in the winter season, and lesions were 1-3 cm in size. Resatorvid Apart from decreasing Cl cases in our study location compared to 2014, building debris around the urban area should be cleaned up concerning standards of hygiene in each phase to keep rodents away. Additionally, citizens at the land border of Iran-Iraq should protect themselves from sand fly biting at least by insect repellent at peak travel times.The role of nitric oxide (NO) in the immunopathological response during Trichinella spiralis (T. spiralis) infection remains controversial. The amino acid, l-arginine is a NO precursor commonly used by athletes and bodybuilders as a protein supplement. As to our knowledge, there are no published studies which have tested the effect of l-arginine on the intestinal phase of experimental trichinellosis. The present work aims to investigate the effect of l-arginine on the enteral phase of experimental T. spiralis infection in albendazole-treated and untreated mice. Forty BALB/C mice infected orally with T. spiralis larvae were divided into 4 groups as follows Group A were infected and untreated (control) mice, Group B received albendazole alone, Group C received l-arginine alone, and Group D received both l-arginine and albendazole. Compared to the control group, l-arginine supplementation showed; a significant increase in the intestinal adult worm burden, a significantly high inducible NO synthase (iNOS) expression, elevated immune markers; tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ), and enhanced apoptosis. Albendazole treated-group had a significant reduction in the adult worm number (90.9%), while combined albendazole-arginine regimen showed a lower percentage of worm reduction (72.7%). During the enteral phase of T. spiralis infection, l-arginine supplementation should be taken cautiously, as it may modulate the proinflammatory immune response and subsequently affect the outcome of the infection and/or treatment.Designing and implementing Cystic Echinococcosis control programs require quantitative information about the worm load and the intensity of infection in dog populations in endemic areas. So far no "probe-based" molecular quantification tool has been available for E. granulosus. This study was conducted in order to develop and evaluate a qPCR technique for measuring worm load of E. granulosus in the final host. A species-specific TaqMan probe was designed based on the available sequences in GenBank. The study was conducted in two stages. First, stool samples from an experimentally infected dog were collected in days 7, 14, 21, 28, 35 and 49, and were analyzed by real-time qPCR assay. In the second stage, 600 mg negative stool specimens were manually spiked with 1, 5, 10, 20 and 40 eggs and the specimens were analyzed using real-time qPCR. According to the standard curve analysis, 93% efficiency and coefficients of correlation (Rsq) > 0.991 were documented. Quantitative PCR assays showed an increasing signal of infection during the 7-week course of infection.