Hsubaker2423

ETHNOPHARMACOLOGICAL RELEVANCE Licorice is an ancient food and medicinal plant. Liquiritigenin and liquiritin, two kinds of major flavonoes in licorice, are effective substances used as antioxidant, anti-inflammatory and tumor-suppressive in food, cosmetics or medicines. However, their in vivo metabolites have not been fully explored. AIM OF STUDY To clarify the metabolism of liquiritigenin and liquiritin in mice. MATERIALS AND METHODS In this study, we developed a liquid chromatography coupled with quadrupole/time-of-flight mass spectrometry method to determine the metabolites in mice plasma, bile, urine and feces after oral administration of liquiritigenin or liquiritin. The structures of those metabolites were tentatively identified according to their fragment pathway, accurate mass, characteristic product ion, metabolism law and reference standard matching. RESULTS A total of 26 and 24 metabolites of liquiritigenin or liquiritin were respectively identified. The products related with apigenin, luteolin or quercetin were the major metabolites of liquiritigenin or liquiritin in mice. Seven main metabolic pathways including (de)hydrogenation, (de)hydroxylation, (de)glycosylation, (de)methoxylation, acetylation, glucuronidation and sulfation were summarized to tentatively explain their biotransformation. CONCLUSION This study not only can provide the evidence for in vivo metabolites and pharmacokinetic mechanism of liquiritigenin and liquiritin, but also may lay the foundation for further development and utilization of liquiritigenin, liquiritin and then licorice. V.OBJECTIVES To evaluate the patterns of use and perceived benefits and barriers among people living with HIV and their physicians concerning telemedicine and the collection of computerized personal information. METHODS Multicenter online observational survey from October 15 to 19, 2018. RESULTS Study participation was accepted by 229 physicians and 838/1,377 PLHIV followed in 46 centers, of which 325 (39%) responded online. We found that while 226/302 (75%) PLHIV accept online prescription renewals and 197/302 (65%) accept online medical certificates, 182/302 (60%) PLHIV-who were more often in material/social deprivation (OR=1.70±0.45; P=0.045), less often born in Île-de-France (OR=0.43±0.15; P=0.018), with lower CD4 T-cell counts (OR=0.999±0.0004; P=0.038), and less often on psychiatric treatment (OR=0.50±0.18; P=0.047)-were receptive to teleconsultations. However, 137/225 (61%) physicians would be uncomfortable teleconsulting due to inadequate data security without it reducing the number of consultations or offering economic benefit. Asked about collection of computerized personal information, 197/296 (67%) PLHIV and 139/223 (62%) physicians agreed it improved quality of care, but 144 (49%) PLHIV and 94/222 (42%) physicians thought it was not sufficiently framed by the law. eHealth was seen as improving coordination between health professionals by 240/296 (81%) PLHIV and seen as a good thing by 181/225 (81%) physicians. CONCLUSION More than half of PLHIV were ready for telemedicine. PLHIV and physicians endorsed the advantage of e-health in terms of better coordination across health professionals but mistrust the data collection factor, which warrants either clarification or stronger legal protections. INTRODUCTION In August 2018 the Ministry of Health of the Democratic Republic of Congo (DRC) declared the 10th epidemic of Ebola virus disease (EVD) in a context of armed conflict and high population mobility. We aimed to assess the knowledge, attitudes, and practices of healthcare professionals related to this disease. METHOD Descriptive and cross-sectional study conducted by self-administered questionnaires among a random sample of 455 healthcare professionals of the city of Butembo, eastern DRC. RESULTS Healthcare professionals were mostly informed about this epidemic by the radio (72.2%).They were usually aware of the disease etiology (65.3%), contamination modes (78.2%), epidemiological surveillance objectives (89%), but they were much less familiar with its clinical outcome (37.9%) and with contact tracing (44.8%). Adequate attitudes and practices were observed in 44.3% of cases for hygiene and sanitation measures, 64.8% of cases for healthcare-associated infection prevention, and 39.7% of cases for the management of probable cases of EVD. CONCLUSION At the start of an Ebola outbreak, capacity building for epidemiological surveillance, hospital hygiene, and safety of care should be among the first urgent actions to implement. The cytoskeleton is a complex network of interconnected biopolymers intimately involved in the generation and transmission of forces. Several mechanical properties of microtubules and actin filaments have been extensively explored in cells. In contrast, intermediate filaments (IFs) received comparatively less attention despite their central role in defining cell shape, motility and adhesion during physiological processes as well as in tumor progression. Here, we explored relevant biophysical properties of vimentin IFs in living cells combining confocal microscopy and a filament tracking routine that allows localizing filaments with ~20 nm precision. A Fourier-based analysis showed that IFs curvatures followed a thermal-like behavior characterized by an apparent persistence length (lp*) similar to that measured in aqueous solution. Additionally, we determined that certain perturbations of the cytoskeleton affect lp* and the lateral mobility of IFs as assessed in cells in which either the microtubule dynamic instability was reduced or actin filaments were partially depolymerized. Our results provide relevant clues on how vimentin IFs mechanically couple with microtubules and actin filaments in cells and support a role of this network in the response to mechanical stress. V.PURPOSE We sought to investigate the performance of high resolution (HR) diffusion-weighted imaging (DWI) using readout-segmented echo-planar imaging (rs-EPI), compared with high-resolution contrast-enhanced MRI (HR CE-MRI) in terms of morphological accuracy, on the basis of the American College of Radiology Breast Imaging and Reporting and Data System (BI-RADS) MRI descriptors and lesion size. selleckchem METHODS This retrospective study included the image data of 94 patients with surgically confirmed malignant breast lesions who had undergone high resolution diffusion-weighted imaging (HR-DWI) and HR CE-MRI. Two radiologists blinded to the final diagnosis independently identified the lesions on HR-DWI, described the morphology of the lesions according to BI-RADS descriptors, and measured lesion size. HR CE-MRI was subsequently evaluated using the same procedure. The inter-method agreement of the morphology and the lesion extent were assessed using kappa statistics. RESULTS Reader A detected 79 mass lesions and 37 non-mass lesions on HR-DWI and HR CE-MRI.