Serupmerritt9737

Hence, the selected E. coli strains exhibited promising probiotic properties with neither enterotoxin nor LPS producibility was detected. The present results confirm the existence of friendly and harmless E. selleck products coli strains in human microbiota as potential probiotics.Probiotic is an alternative method to treat intestinal infection disease caused by antibiotic-resistant bacteria. In this study, Lactococcus lactis KA-FF 1-4 demonstrated to have the potential to inhibit the growth of Vancomycin-resistant enterococci (VRE) by producing anti-microbial substance. In co-culture, L. lactis KA-FF 1-4 (108 CFU/mL) inhibited the growth of VRE from 103-104 CFU/mL to zero after 6 h of exposure. However, in a gut model contained human gut microbiota, this anti-VRE activity of L. lactis KA-FF 1-4 was reduced to only 3.59-6.12%. The unexpected difference in efficacy between the experimental models could be explained by the fact that the growth of L. lactis KA-FF 1-4 was stable in the gut model. Leaving aside these limitations, we observed that adding L. lactis KA-FF 1-4 into the human gut model containing VRE was able to enhance microbial richness and diversity. Specifically, a higher abundance of beneficial microbes from the group of Bifidobacterium spp. and Bacteroides fragilis. L. lactis KA-FF 1-4 also enhanced the abundance of Parabacteroides, Lactococcus, and Fusobacterium and promoted the production of lactic acid in the gut model. However, these effects were not observed in the gut model without L. lactis KA-FF 1-4. Even though this study could not demonstrate a significant anti-VRE effect of the L. lactis KA-FF 1-4 in a gut model, our results still offer evidence that L. lactis KA-FF 1-4 could positively modulate the gut microbiota by promoting the growth of beneficial microbes and their metabolite. L. lactis KA-FF 1-4 has probiotic properties to fight against VRE infection, therefore further investigation in animal model is needed.In this study, the ultrasound-assisted surfactant aqueous system coupled with microbial consortia immobilized by cellulose has been created as an enhanced and sustainable method for the bioconversion and extraction of resveratrol from peanut skin. Based on central composite design, and several single-factor experiments, we derived the optimal bioconversion and extraction system. Microbial consortia consist of Yeast CICC 1912, Aspergillus oryzae 3.951 and Aspergillus niger 3.3148 were chosen to be immobilized using cellulose. Other treatment conditions include concentration of surfactant as 3% (w/v), temperature as 30 °C, time as 36 h, ultrasonic power as 250 W and liquid to solid ratio as 251 mL/g. Under these conditions, we achieved a promising yield of resveratrol 96.58 μg/g, which is 4.02 folds compared to the untreated sample. This sustainable and green method not only enhanced the production of resveratrol but also improved the safety and reliability of the bioconversion and extraction process. Our novel method has shown great potential to realize large-scale bioconversion and extraction of bioactive compounds from plant waste.Stem cells are becoming increasingly popular in public lexicon owing to their prospective applications in the biomedical and therapeutic domains. Extensive research has found various independent stem cell systems fulfilling specific needs of plant development. Plant stem cells are innately undifferentiated cells present in the plant's meristematic tissues. Such cells have various commercial uses, wherein cosmetic manufacture involving stem cell derivatives is the most promising field at present. Scientific evidence suggests anti-oxidant and anti-inflammatory properties possessed by various plants such as grapes (Vitis vinifera), lilacs (Syringa vulgaris), Swiss apples (Uttwiler spatlauber) etc. are of great importance in terms of cosmetic applications of plant stem cells. There are widespread uses of plant stem cells and their extracts. The products so formulated have a varied range of applications which included skin whitening, de-tanning, moisturizing, cleansing etc. Despite all the promising developments, the domain of plant stem cells remains hugely unexplored. This article presents an overview of the current scenario of plant stem cells and their applications in humans.Differential transcriptome analysis is an effective method for gene selection of triterpene saponin biosynthetic pathways. MeJA-induced differential transcriptome of Panax notoginseng has not been analyzed yet. In this study, comparative transcriptome analysis of P. notoginseng roots and methyl jasmonate (MeJA)-induced roots revealed 83,532 assembled unigenes and 21,947 differentially expressed unigenes. Sixteen AP2/ERF transcription factors, which were significantly induced by MeJA treatment in the root of P. notoginseng, were selected for further analysis. Real-time quantitative PCR (RT-qPCR) and co-expression network analysis of the 16 AP2/ERF transcription factors showed that PnERF2 and PnERF3 had significant correlation with dammarenediol II synthase gene (DS) and squalene epoxidase gene (SE), which are key genes in notoginsenoside biosynthesis, in different tissues and MeJA-induced roots. A phylogenetic tree was conducted to analyze the 16 candidate AP2/ERF transcription factors and other 38 transcription factors. The phylogenetic tree analysis showed PnERF2, AtERF3, AtERF7, TcERF12 and other seven transcriptional factors are in same branch, while PnERF3 had close evolutionary relationships with AtDREB1A, GhERF38 and TcAP2. The results of comparative transcriptomes and AP2/ERF transcriptional factors analysis laid a solid foundation for further investigations of disease resistance and notoginsenoside biosynthesis in P. notoginseng.In the present study, the diversity of 11 Thymus species was assessed using molecular and morphological markers. Essential oil content and morphological traits were also investigated during two agronomic years. The result of the analysis of variance showed considerable differences among morphological traits. In the first and second years, the essential oil content of the species varied from 0.63 to 1.94% and 0.86 to 2.34%, respectively. T. vulgaris and T. migricus showed the highest essential oil content in two agronomic years. In this research, nine ISSR primers were also used to amplify 151 polymorphic bands in 77 accessions belonging to 11 Thymus species. Cluster and principal component (PCA) analyses classified the species in three major groups. Among the species, T. vulgaris and T. fedtschenkoi presented relatively higher genetic distance in comparison with other species. Analysis of molecular variance (AMOVA) revealed that 72.34% of the total variation was belonged to within-species variation, while 27.