Halseyholgersen6408

Barite (BaSO4) is a component of drilling fluids used in the oil and gas industry and may cause barium (Ba) contamination if it is spilled onto flooded soils. Under anoxic soil conditions and low redox potential, sulfate can be reduced to a more soluble form (sulfide), and Ba can be made available. To design a solution for such environmental issues, a field study was conducted in a Ba-contaminated flooded area in Brazil, in which we induced Ba phytoextraction from the management of the planting density of two intercropped macrophytes. Typha domingensis and Eleocharis acutangula were grown in four initial planting densities "Ld" (low density 4 and 32 plants m-2); "Md" (medium density 8 and 64 plants m-2); "Hd" (high density 12 and 128 plants m-2); "Vhd" (very high density 16 and 256 plants m-2). Vhd produced the largest number of plants after 300 days. However, the treatments did not differ in terms of the amount of biomass. The increments in the initial planting density did not increase the Ba concentration in the aerial part. The greatest Ba phytoextraction (aerial part + root) was achieved by Ld treatment, which removed approximately 3 kg of Ba ha-1. Md and Vhd treatments had the highest Ba translocation factors. Because more plants per area did not result in greater Ba phytoextraction, a lower planting density was recommended for the intercropping of T. domingensis and E. acutangula to promote the phytoextraction of barium, due to possible lower implementation costs in contaminated flooded environments.Honey bees are important pollinators and are subject to numerous stressors, such as changing floral resources, parasites, and agrochemical exposure. Pesticide exposure has been linked to the decline in the global honey bee population. We have limited knowledge of the metabolic pathways and synergistic effects of xenobiotics in bees. Quercetin is one of the most abundant phytochemicals in plants and is therefore abundant in the honey bee diet. Quercetin can upregulate the detoxification system in honey bees; however, it is still unknown to what extent quercetin ingestion can reduce the content of absorbed pesticides. In this study, we investigated the effect of dietary quercetin on the contents of three pesticides in honey bees imidacloprid (insecticide), tebuconazole (fungicide), and tau-fluvalinate (insecticide and acaricide). Bees were divided into two main groups and fed either quercetin-sucrose paste or only sucrose for 72 h. Thereafter, they were orally exposed to ∼10 ng/bee imidacloprid or contact-exposed to ∼0.9 μg/bee tau-fluvalinate or ∼5.2 μg/bee tebuconazole. After 1 h of oral exposure or 24 h of contact exposure, the bees were anaesthetised with CO2, sacrificed by freezing, and extracted with a validated QuEChERS method. Subsequently, the concentrations of the three pesticides and quercetin in the bees were determined with a triple quadrupole tandem mass spectrometer coupled to an HPLC system. No significant effect on the concentration of tebuconazole or tau-fluvalinate was observed in bees fed quercetin. Intake of quercetin led to a reduction in the concentration of imidacloprid in honey bees. Quercetin-rich plants may be exploited in future beekeeping.Yellow mealworms (Tenebrio molitor larvae) are capable of biodegrading polystyrene (PS) and low-density polyethylene (LDPE). This study tested biodegradation of one expanded PS (EPS) with a weight-average molecular weight (Mw) 256.4 kDa and two LDPE foams with respective Mw of 130.6 kDa (PE-1) and 288.7 kDa (PE-2) in T. monitor larvae obtained in Beijing, China. The larvae consumed EPS and both LDPEs over a 60 day. Fourier transform infrared spectroscopy and thermogravimetric analyses of frass confirmed the formation of new oxygen-containing functional groups, as well as a change in physical property and chemical modification, indicating that biodegradation of EPS and LDPE occurred. Gel permeation chromatography analysis confirmed broad depolymerization of EPS and PE-1 (i.e., a decrease in both Mw and a number-average molecular weight (Mn)) but revealed limited extent depolymerization of PE-2 (i.e., increase in Mn and decrease in Mw). For all materials, the size-average molecular weight (Mz) was decreased. Biodegradation and oxidation of EPS and LDPE were confirmed using FTIR and TGA analysis. Depression of gut microbes by the antibiotic gentamicin resulted in significant inhibition of EPS depolymerization but did not stop LDPE depolymerization, resulting in the increase in Mn and revealing that PS biodegradation was gut microbe-dependent but LDPE biodegradation was less dependent or independent of gut microbes. Gut microbial community analysis indicated that, as expected, under different dietary conditions, the intestinal flora significantly shifted to communities associated with biodegradation of EPS and LDPE. The results indicated the complexity and limitation of biodegradation of plastics in plastics-eating T. molitor larvae.Frequent drying and rewetting due to flooding/precipitation and drainage events in floodplains induces changes in biogeochemical conditions that may influence the effectiveness of in situ Hg stabilization using biochars as soil amendments. This study evaluated two selected biochars anaerobic digestate (DIG) and sulfurized hardwood (MOAK)) as potential amendment materials in moderately reduced floodplain soil under repeated drying and rewetting events using a modified humidity cell protocol. see more Enhanced release of filter-passing (0.45-μm) total Hg (THg) and MeHg was observed at early times. Elevated concentrations of 0.45-μm THg were associated with DOC and Mn in sediment control and biochar-amended systems. Elevated concentrations of MeHg were associated with Mn in the MOAK-amended system. Thereafter, decreases in 0.45-μm (up to 57%) and unfiltered THg (up to 93%) were observed. As wetting and drying events continued, decreases in pH and alkalinity as well as increases in SO42- (up to 796 mg L-1) and Ca (up to 215 mg L-1) were observed in the MOAK-amended systems with the microbial community shifted towards sulfur-oxidizing bacteria, indicating microbially-driven oxidation of MOAK. Although results of S K-edge X-ray absorption near edge structure (XANES) analysis suggest polysulfur is the predominant S phase in both MOAK- and DIG-amended systems, microbially-driven oxidation of DIG was not observed. Polysulfur in MOAK from the sulfurization process is more bioavailable to sulfur oxidizing communities than in DIG under the repeated drying and wetting conditions. Results of this study suggest biogeochemical conditions as well as biochar properties should be considered when planning full-scale field applications.