Hovgaardhoffman1945

OBJECTIVE This study aimed to explore whether exposure to ceftriaxone during early life could influences glucose and lipid metabolism of high fat diet-induced mice. METHODS Total 48 of female BALB/c aged 2 week old were randomly divided into control group(treated with saline), antibiotic group(treated with100 mg/kg ceftriaxone), high-fat diet group(treated with saline) and combined action group(treated with 100 mg/kg ceftriaxone)(n=12), respectively to stop gavage 2 weeks later. Then high-fat diet group and combined action group were fed with high-fat diet for 12 weeks. Fasting blood glucose(FBG) and oral glucose tolerance test were conducted in the last week. Serum total cholesterol(TC), triglyceride(TG), high-density lipoprotein cholesterol(HDL-C), low-density lipoprotein cholesterol(LDL-C), fasting insulin, leptin and TG, TC in liver were also measured. Furthermore, homeostasis model assessment of insulin resistance(HOMA-IR) was calculated from FBG and insulin. RESULTS Compared with normal chow diet, high-fat diet impaired oral glucose tolerance and increased the levels of abdominal adipose tissue, FBG, HOMA-IR, lips in serum and liver and leptin(P less then 0. 05). The oral administration of ceftriaxone in early life impaired oral glucose tolerance and increased the levels of abdominal adipose tissue, FBG and TG in liver(P less then 0. 05). In addition, early ceftriaxone intervention could enhance the impaired glucose tolerance, the increasing FBG, insulin resistance and liver lipids associated with high-fat diet(P less then 0. 05). CONCLUSION Early ceftriaxone intervention not only significantly increases the level of abdominal adipose tissue, FBG, insulin resistance and liver lipids, but also enhances glycolipid metabolic disorders induced by high-fat diet. These result suggest that the exposure to antibiotics in the early life might increase the sensitivity of host animal to high fat diet induced abnormal glycolipid metabolism late.OBJECTIVE To investigate effects of different ratios of n-6/n-3 Polyunsaturated fatty acid(PUFA) on adiponectin, glycolipid metabolism and antioxidant capacity in high fat-diet fed rats. METHODS Fixty male Wistar rats were randomly divided into control group(CON, 17% energy from fat, n-6/n-3 PUFA=5∶1) and high-fat treatment groups(1∶1 group∶n-6/n-3 PUFA=1∶1, 5∶1 group, n-6/n-3 PUFA=5∶1, 10∶1 group∶ n-6/n-3 PUFA=10∶1 and 20∶1 group∶ n-6/n-3 PUFA=20∶1, 42% energy from fat). Rats were fed for 12 week. Serum glucose and lipid(0, 4, 8 and 12 week) were detected. Antioxidant indexes(0 and 12 week), adiponectin mRNA and protein expressions in epididymal adipose tissues as well as serum adiponectin(12 week) were measured. RESULTS Baseline data showed no significant inter-group difference(P>0. 05). 1∶1 and 5∶1 showed no significant changes in serum glucose compared with control at all time points(P>0. 05), while 10∶1 and 20∶1 significantly increased it at the 12 th week(P less then 0. 05). At the 12 th week, total choent of adiponectin expression of rats fed with high-fat diet.OBJECTIVE To establish an rat basophil leukemia(RBL)-2H3 cell line stably expressing human high affinity receptor containing alpha, beta and gamma chain(hFcεRIαβγ), in order to provide experimental materials for evaluating allergenicity of food. METHODS The lentivirus was transfected into RBL-2H3 cells, and the mRNA expression of hFcεRIαβγ in cells was detected by real-time PCR and the protein expression of hFcεRIα was detected by flow cytometry. RESULTS Sequencing result showed that recombinant lentiviral vector GV367-hFcεRIαβγ was successfully constructed. According to the result of experiments, lentivirus could effectively infect RBL-2H3 cells. The mRNA of hFcεRIαβγ and protein levels of hFcεRIα in RBL-2H3 cells were successfully overexpressed. CONCLUSION The hFcεRIαβγ/RBL-2H3 cells were preliminarily constructed, which could be binded with human IgE and further used in the evaluation system of food allergy, compared to RBL-2H3 cells.OBJECTIVE To master the variation characteristics of PM_(2. 5) mass concentration in Lianhu district and Yanta district of Xi'an City and its relationship with meteorological conditions. METHODS From 2015 to 2018, according to the environmental monitoring data of six main urban areas in Xi'an City in 2012, including NO_2, SO_2, PM_(10), PM_(2. 5), CO and O_3, air samples were collected in the relatively heavy polluted Lianhu district and the relatively light Yanta district of Xi'an City. The mass concentration test of PM_(2. 5) was carried out in accordance with the Ministry of Environmental Protection's "Determination of atmospheric articles PM_(10) and PM_(2. 5) in ambient air by gravimetric method "(HJ 618-2011). According to the Ambient air quality standards(GB 3095-2012), the average daily secondary concentration limit(75 μg/m~3) was used for statistical analysis and evaluation according to different annual, regional and seasonal test result. buy SAR405838 Meteorological data of Xi'an City werad improved year by year, and the pollution of PM_(2. 5) in autumn and winter was more serious. Meteorological conditions affected the concentration level of PM_(2. 5) in the atmosphere.OBJECTIVE To understand the occupational stress and sub-health level of pharmaceutical marketers in Northeast China, and to analyze their influencing factors and the impact of occupational stress on sub-health. METHODS From October to December in 2016, we conducted a questionnaire survey about occupational stress and sub-health status of 698 women and 454 men pharmaceutical marketers aged from 22 to 58 years old in northeast China. RESULTS The rate of occupational stress was 86. 9%. The top 3 stressors with higher scores were the pressure brought about by the intensification of social competition(2. 90±1. 017), the higher standards of job assessment and the great efforts needed by individuals(2. 90±0. 959). The detection rate of sub-health was 82. 1%. There were statistically differences in the rate of sub-health detection among the groups of different genders(χ~2=4. 726, P=0. 030) and different business types(χ~2=7. 961, P=0. 047). There were statistically significant differences in the rate of different ages(χ~2=9.