Tierneysmith4952

RESULTS The food intake and body weight of rats exposed to CIS were lower than those of control rats. The serum leptin, and expression levels of Ob-R, POMC and α-MSH in the ARC were significantly higher than those in the control rats. Treatment with the XYS decoction improved the appetite and the body weight, and down-regulated serum leptin and Ob-R, POMC and α-MSH in hypothalamus ARC. CONCLUSION The leptin-Ob-R-POMC pathway might be the part of the mechanism underlying XYS's improvement of somatic symptoms such as reduction in food intake and body weight related to CIS.OBJECTIVE To observe the effect of Yanggan Jiedu Sanjie (YGJDSJ) formula on human hepatocellular carcinoma Bel-7402 cells. METHODS Bel-7402 cells were treated with YGJDSJ. Cell proliferation was detected by cell counting kit-8 assay. Cell apoptosis was identified by Hoechst 33258 staining and flow cytometric analysis. Cell cycle distribution was quantified by flow cytometric analysis. Caspase activities were measured by commercial kit. Y-27632 clinical trial Cell senescence was detected by senescence-associated β-galactosidase (SA-β-gal) staining. Protein expression and phosphorylation were identified by Western blot. Protein expression was knocked-down by siRNA. RESULTS YGJDSJ inhibited proliferation of Bel- 7402 cells in a dose- and time-dependent manner. YGJDSJ induced apoptosis and activated caspase- 3, 8, and 9 in Bel-7402 cells. YGJDSJ-induced apoptosis was completely abrogated by a pan caspase inhibitor, Z-VAD-FMK. YGJDSJ also induced cell senescence, up-regulated cyclin-dependent kinase inhibitor 1a (CDKN1a) and CDKN2a expression and down-regulated retinoblastoma protein (RB) phosphorylation in Bel-7402 cells. Specific knockdown of CDKN1a and CDKN2a significantly reduced YGJDSJ-induce cell senescence in Bel-7402 cells. CONCLUSION YGJDSJ inhibited cell proliferation, induced caspase-dependent apoptosis and CDKN1a/CDKN2a-RB signalling mediated cell senescence in Bel-7402 cells. Our findings suggest that YGJDSJ might be potential for hepatocellular carcinoma treatment.OBJECTIVE To find out the combination of the extracts from Paeonia lactiflora Pallas (PL), Reh- mannia Glutinosa var. Purpurea Makino (RG), Perilla Frutescens var. Acuta Kudo (PF) to increase endometrial receptivity. METHODS Herbal medicines were extracted with boiling water and polysaccharides were removed. We examined the effect of PL, RG, and PF (PRP), a most effective herbal formula deduced from constitutive ingredient herbs of Antai Yin which is composed of PRP, on the leukemia inhibitory factor (LIF) expression and endometrial receptivity. RESULTS The combination of the extracts from PRP induced the LIF expression in Ishikawa cells and increased the adhesion between Ishikawa and JAr cells. In addition, PRP-induced attachment of JAr cells onto Ishikawa cells and expression of adhesion molecules, ITGAV, ITGB5, CD44s, and L-selectin, are significantly reduced by knock-down of LIF expression. CONCLUSION Induced by the combination of the PRP extracts, the adhesion between trophoblast and endometrial cells are mediated by expression of LIF and adhesion molecules. Thus, we suggest the combination of the PRP extracts may be a novel therapy for enhancing embryo implantation rate.OBJECTIVE To evaluate current evidence on the efficacy and safety of Cordyceps sinensis (cordyceps) or its fermented products used as an adjunctive treatment in patients undergoing maintenance hemodialysis. METHODS The Cochrane Central Register of Controlled Trials (CENTRAL), EMBASE, MEDLINE, China National Knowledge Infrastructure (CNKI) and Wanfang Database were searched for relevant randomized controlled trials up to March 2016. Two review authors independently selected trials for inclusion, extracted data, assessed the methodological quality and rated the quality of evidence with the Grading of Recommendations, Assessment, Development and Evaluation approach. RESULTS Twelve studies involving 655 participants were included. Evidence of low to moderate-quality showed that cordyceps plus conventional treatment compared to conventional treatment alone significantly improved C-reactive protein [standardized mean difference (SMD) －0.61; 95% confidence intervals (CI) －1.00 to －0.22], high-sensitivity C-reactive protein [weighted mean difference (WMD) －3.44 mg/L; 95% CI －3.89 to －2.99], serum albumin (WMD 3.07 g/L; 95% CI 1.59 to 4.55), malondialdehyde (WMD －1.95 nmol/L; 95% CI －2.24 to －1.66), and hemoglobin (WMD 9.56 g/L; 95% CI 3.65 to 15.47) levels. However, there was no significant improvement for serum creatinine and low-density lipoprotein cholesterol. Overall, most trials either did not monitor adverse events or poorly documented them. CONCLUSION Given the small number of trials included, the unclear methodological quality of the included trials, and the high heterogeneity in pooled analyses, the evidence obtained in this review is insufficient to recommend the use of cordyceps as adjunctive treatment in hemodialysis patients.OBJECTIVE To research the Traditional Chinese Medicine (TCM) practice of Professor Xu Runsan for treatment of chronic pelvic pain (CPP) caused by sequelae of pelvic inflammatory disease (SPID) by data mining. METHODS The medical records of inpatients at China-Japan Friendship Hospital confirmed to have CPP caused by SPID were collected (274 visits in total). The data extracted from the medical records were analyzed by frequency statistics, correlation analyses, cluster analyses, and complex network analyses. RESULTS The most frequently used medicines were warm medicines, bitter medicines, and medicines distributed to the liver meridian. The most common medicinal combinations were Chishao (Radix Paeoniae Rubra) plus Huangqi (Radix Astragali Mongolici) plus Sanqi (Radix Notoginseng) and Ezhu (Rhizoma Curcumae Phaeocaulis); Guizhi (Ramulus Cinnamomi) plus Fuling (Poria) and Chishao (Radix Paeoniae Rubra); and Chaihu (Radix Bupleuri Chinensis) plus Zhishi (Fructus Aurantii Immaturus) and Gancao (Radix Glycyrrhizais prescriptions are often based on Guizhi Fuling Pill and Sini Powder. More blood-activating, stasis-resolving, or tonifying medicines could be used according to the accompanying symptoms or symptom patterns identified.