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Transition metal ions (Fe, Mn, Cu, Zn) are essential for healthy brain function, but altered concentration, distribution, or chemical form of the metal ions has been implicated in numerous brain pathologies. Currently, it is not possible to image the cellular or sub-cellular distribution of metal ions in vivo and therefore, studying brain-metal homeostasis largely relies on ex vivo in situ elemental mapping. Sample preparation methods that accurately preserve the in vivo elemental distribution are essential if one wishes to translate the knowledge of elemental distributions measured ex vivo toward increased understanding of chemical and physiological pathways of brain disease. The choice of sample preparation is particularly important for metal ions that exist in a labile or mobile form, for which the in vivo distribution could be easily distorted by inappropriate sample preparation. One of the most widely studied brain structures, the hippocampus, contains a large pool of labile and mobile Zn. Herein, we describe how sucrose cryoprotection, the gold standard method of preparing tissues for immuno-histochemistry or immuno-fluorescence, which is also often used as a sample preparation method for elemental mapping studies, drastically alters hippocampal Zn distribution. Based on the results of this study, in combination with a comparison against the strong body of published literature that has used either rapid plunge freezing of brain tissue, or sucrose cryo-protection, we strongly urge investigators in the future to cease using sucrose cryoprotection as a method of sample preparation for elemental mapping, especially if Zn is an analyte of interest.Income inequality among U.S. families with children has increased over recent decades, coinciding with a period of significant reforms in federal welfare policy. In the most recent reform eras, welfare benefits were significantly restructured and redistributed, which may have important implications for income inequality. Using data from the 1968-2016 March Supplement to the Current Population Survey (N = 1,192,244 families with children) merged with data from the historical Supplemental Poverty Measure, this study investigated how income inequality and, relatedly, the redistributive effects of welfare income and in-kind benefits changed, and whether such changes varied across states with different approaches to welfare policy. Results suggest that cash income from welfare became less effective at reducing income inequality after the 1996 welfare reform, because the share of income coming from cash welfare fell and was also less concentrated among the neediest families. At the same time, tax and in-kind benefits reduced inequality until the Great Recession. Consistent with the "race to the bottom" hypothesis, results suggest that the redistributive effects of welfare income dropped in all states regardless of their approach to welfare policy.We use experimental and survey measures to evaluate the time and risk preferences of nearly 500 adolescents aged 16-19 years old. We find that survey questions about time and risk preferences are weakly correlated with corresponding experiments in which participants trade-off monetary rewards. We find potentially substantial inter-generational transfer of time and risk preferences parent time and risk preferences are strongly predictive of adolescent preferences for both survey and experimental measures. There are also interesting heterogeneities girls are less risk seeking and more patient than boys when risk and time preferences are measured via surveys. Interestingly, the survey measures have more predictive power for field outcomes than the experimental measures. Higher patience as measured by the survey is significantly associated with lower body mass index (BMI), less time spent on sedentary activities, more time spent on physical activity and lower consumption of fast food and sweets.Pelvic fractures occur in up to 25% of all severely injured trauma patients and its mortality is markedly high despite advances in resuscitation and modernization of surgical techniques due to its inherent blood loss and associated extra-pelvic injuries. Pelvic ring volume increases significantly from fractures and/or ligament disruptions which precludes its inherent ability to self-tamponade resulting in accumulation of hemorrhage in the retroperitoneal space which inevitably leads to hemodynamic instability and the lethal diamond. Pelvic hemorrhage is mainly venous (80%) from the pre-sacral/pre-peritoneal plexus and the remaining 20% is of arterial origin (branches of the internal iliac artery). This reality can be altered via a sequential management approach that is tailored to the specific reality of the treating facility which involves a collaborative effort between orthopedic, trauma and intensive care surgeons. Tie2 kinase inhibitor 1 We propose two different management algorithms that specifically address the availability of qualified staff and existing infrastructure one for the fully equipped trauma center and another for the very common limited resource center.Pancreatic trauma is a rare but potentially lethal injury because often it is associated with other abdominal organ or vascular injuries. Usually, it has a late clinical presentation which in turn complicates the management and overall prognosis. Due to the overall low prevalence of pancreatic injuries, there has been a significant lack of consensus among trauma surgeons worldwide on how to appropriately and efficiently diagnose and manage them. The accurate diagnosis of these injuries is difficult due to its anatomical location and the fact that signs of pancreatic damage are usually of delayed presentation. The current surgical trend has been moving towards organ preservation in order to avoid complications secondary to exocrine and endocrine function loss and/or potential implicit post-operative complications including leaks and fistulas. The aim of this paper is to propose a management algorithm of patients with pancreatic injuries via an expert consensus. Most pancreatic injuries can be managed with a combination of hemostatic maneuvers, pancreatic packing, parenchymal wound suturing and closed surgical drainage. Distal pancreatectomies with the inevitable loss of significant amounts of healthy pancreatic tissue must be avoided. General principles of damage control surgery must be applied when necessary followed by definitive surgical management when and only when appropriate physiological stabilization has been achieved. It is our experience that viable un-injured pancreatic tissue should be left alone when possible in all types of pancreatic injuries accompanied by adequate closed surgical drainage with the aim of preserving primary organ function and decreasing short and long term morbidity.